Alternatively, the stock tradition organisms can be developed in a suitable liquid medium (i.e., Soybean&#one hundred fifty;Casein Digest Broth or Sabouraud Dextrose Broth) plus the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to obtain a microbial count of about 1 × 108 cfu for every mL. [Notice—The est
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